Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MafA Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435).

Reactivity Notes

Human reactivity reported in the scientific literature (PMID: 22665223).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MafA Antibody

Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137] - Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Affinity purified anti-MafA antibody (NB400-137), anti-MafB antibody (NB600-266) or anti-c-Maf antibody (NB600-267). Each antibody was used at 0.5 ug/ml.
Immunohistochemistry-Paraffin: MafA Antibody [NB400-137]

Immunohistochemistry-Paraffin: MafA Antibody [NB400-137]

Immunohistochemistry-Paraffin: MafA Antibody [NB400-137] - Immunohistochemical staining of MafA (blue) and insulin (green) in mouse pancreas. Image from verified customer review.
Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137] - Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody (NB 400-137).
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Applications for MafA Antibody

Application
Recommended Usage

Gel Super Shift Assays

1 to 5 ug/20 ul reaction

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000 to 1:3000

Western Blot

1:1000-1:10000
Application Notes
A band ~36-40 kDa is seen in WB analysis. Use in IHC-P was reported in the scientific literature (PMID: 22665223). In some cases, the antibody may be diluted further than indicated. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in ICC/IF and in IHC-Frozen reported in scientific literature (PMID 23967072). Chip reported in scientific literature (PMID: 26314560).

Reviewed Applications

Read 1 review rated 5 using NB400-137 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MafA

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Alternate Names

hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3B1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)

Entrez Gene IDs

378435 (Mouse)

Gene Symbol

MAFA

UniProt

Q8CF90 (Mouse)

Additional MafA Products

Product Documents for MafA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MafA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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