MafG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15019

Novus Biologicals, part of Bio-Techne
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NBP2-15019
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (98%), Chicken (92%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MafG Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33754066). Zebrafish reactivity reported in scientific literature (PMID: 27737822).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MafG Antibody

Western Blot: MafG Antibody [NBP2-15019]

Western Blot: MafG Antibody [NBP2-15019]

Western Blot: MafG Antibody [NBP2-15019] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MafG Antibody [NBP2-15019]

Immunocytochemistry/ Immunofluorescence: MafG Antibody [NBP2-15019]

Immunocytochemistry/Immunofluorescence: MafG Antibody [NBP2-15019] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG protein stained by MAFG antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.
Immunohistochemistry-Paraffin: MafG Antibody [NBP2-15019]

Immunohistochemistry-Paraffin: MafG Antibody [NBP2-15019]

Immunohistochemistry-Paraffin: MafG Antibody [NBP2-15019] - PC9 xenograft, using MAFG antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for MafG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
Chip Assay, Chip-seq-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MafG

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog G

Alternate Names

basic leucine zipper transcription factor MafG, hMAF, MGC13090, MGC20149, transcription factor MafG, v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)

Gene Symbol

MAFG

UniProt

O15525

Additional MafG Products

Product Documents for MafG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MafG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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