MAGEA1 Antibody (MZ2E/838)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44508
![Immunohistochemistry-Paraffin: MAGEA1 Antibody (MZ2E/838) [NBP2-44508]](https://resources.bio-techne.com/images/products/MAGE-1-Antibody-MZ2E-838-Immunohistochemistry-Paraffin-NBP2-44508-img0001.jpg "Immunohistochemistry-Paraffin: MAGEA1 Antibody (MZ2E/838) [NBP2-44508]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MZ2E/838
Concentration
0.2 mg/ml
Product Summary for MAGEA1 Antibody (MZ2E/838)
Immunogen
Recombinant human MAGEA1 protein (Uniprot: P43355)
Localization
Cytoplasmic
Specificity
Recognizes a protein of 42-46kDa, identified MAGEA1 (MAGE-1). This monoclonal antibody does not cross-react with other members of MAGE-family. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGEA1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11463)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for MAGEA1 Antibody (MZ2E/838)
Immunohistochemistry-Paraffin: MAGEA1 Antibody (MZ2E/838) [NBP2-44508]
Immunohistochemistry-Paraffin: MAGE 1 Antibody (MZ2E/838) [NBP2-44508] - Human Testicular Carcinoma stained with MAGE-1 Monoclonal Antibody (MZ2E/838).Applications for MAGEA1 Antibody (MZ2E/838)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MAGEA1
Long Name
Melanoma-Associated Antigen 1
Alternate Names
CT1.1, MAGE-A1, MAGE1, MGC9326, MZ2-E
Gene Symbol
MAGEA1
UniProt
Additional MAGEA1 Products
Product Documents for MAGEA1 Antibody (MZ2E/838)
Product Specific Notices for MAGEA1 Antibody (MZ2E/838)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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