MAGEA2 Antibody (1H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00004101-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAGEA2 Antibodies »

Product Specifications

Immunogen

MAGEA2 (AAH63681, 1 a.a. ~ 314 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQQTASSSSTLVEVTLGEVPAADSPSPPHSPQGASSFSTTINYTLWRQSDEGSSNQEEEGPRMFPDLESEFQAAISRKMVELVHFLLLKYQAREPVTKAEMLESVLRNCQDFFPVIFSKASEYLQLVFGIEVVEVVPISHLYILVTCLGLSYDGLLGDNQVMPKTGLLIIVLAIIAIEGDCAPEEKIWEELSMLEVFEGREDSVFAHPRKLLMQDLVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHTLKIGGEPHISYPPLHERALREGEE

Specificity

MAGEA2 - melanoma antigen family A, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MAGEA2 Antibody (1H4)

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01]

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01]

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01] - Analysis of MAGEA2 expression in transfected 293T cell line by MAGEA2 monoclonal antibody (M01), clone 1H4.Lane 1: MAGEA2 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.
Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01]

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01]

Western Blot: MAGEA2 Antibody (1H4) [H00004101-M01] - MAGEA2 monoclonal antibody (M01), clone 1H4 Analysis of MAGEA2 expression in Jurkat.
Sandwich ELISA: MAGEA2 Antibody (1H4) [H00004101-M01] - Detection limit for recombinant GST tagged MAGEA2 is 0.03 ng/ml as a capture antibody.

Applications for MAGEA2 Antibody (1H4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAGEA2

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene.

Alternate Names

Cancer/testis antigen 1.2, CT1.2MGC131923, MAGE-2 antigen, MAGEA2Amember 2, MAGEA2B, melanoma antigen 2, melanoma antigen family A, 2, melanoma-associated antigen 2

Entrez Gene IDs

4101 (Human)

Gene Symbol

MAGEA2

OMIM

300173 (Human)

UniProt

AAH63681 (Human)

Additional MAGEA2 Products

Product Documents for MAGEA2 Antibody (1H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAGEA2 Antibody (1H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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