MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20167
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 21B4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3.
Specificity
This antibody binds human MAGEA3 and MAGEA6, but does not cross react with other members of the MAGE family like MAGE-A1, MAGE-A4 and MAGE-A10.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free
Western Blot: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] -
Western Blot: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - HeLa(A) (0.01ug/ml) cells lysate and HeLa(B) (0.01ug/ml) cells nuclear lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 21B4 (NBP3-20167) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] -
Immunocytochemistry/Immunofluorescence: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21B4 (NBP3-20167) (1:1000 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20167, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (NBP2-52638) followed by staining with Alexa Fluor(R) 488 secondary antibody.
Flow Cytometry: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] -
Flow Cytometry: MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free [NBP3-20167] - HeLa cells were stained with an isotype control (NBP2-52638, black line) or the rabbit-chimeric version of 21B4 (NBP3-20167, blue line) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488(R) conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.Applications for MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: MAGEA3
Long Name
Melanoma-Associated Antigen 3
Alternate Names
CT1.3, MAGE3
Gene Symbol
MAGEA3
Additional MAGEA3 Products
Product Documents for MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free
Product Specific Notices for MAGEA3 Antibody (21B4) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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