Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1G9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MAGEA3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MAGEA3 (NP_005353) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

34.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAGEA3 Antibody (OTI1G9) - Azide and BSA Free

Western Blot: MAGEA3 Antibody (OTI1G9)Azide and BSA Free [NBP2-72570]

Western Blot: MAGEA3 Antibody (OTI1G9)Azide and BSA Free [NBP2-72570]

Western Blot: MAGEA3 Antibody (OTI1G9) - Azide and BSA Free [NBP2-72570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3.
Western Blot: MAGEA3 Antibody (OTI1G9)Azide and BSA Free [NBP2-72570]

Western Blot: MAGEA3 Antibody (OTI1G9)Azide and BSA Free [NBP2-72570]

Western Blot: MAGEA3 Antibody (OTI1G9) - Azide and BSA Free [NBP2-72570] - Analysis of extracts (10ug) from MCF7 cell line.

Applications for MAGEA3 Antibody (OTI1G9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAGEA3

MAGEA3, also known as Melanoma-associated antigen 3, is a 34.7 kDa, 314 amino acid protein that is involved in increasing ubiquitin ligase activity as a member of the MAGEA family. Current research is being conducted to study the effects of the protein on diseases and disorders such as bile duct cancer, dyskeratosis, ocular melanoma, germ cell tumors, multiple types of carcinomas, avian influenza, testicular cancer, lung cancer, and lymphoma. The protein interacts with other proteins such as TRIM28, UBC, HTT, and SUMO2.

Long Name

Melanoma-Associated Antigen 3

Alternate Names

CT1.3, MAGE3

Gene Symbol

MAGEA3

Additional MAGEA3 Products

Product Documents for MAGEA3 Antibody (OTI1G9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAGEA3 Antibody (OTI1G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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