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MAGOH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97072

Novus Biologicals, part of Bio-Techne
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NBP2-97072-100ul
NBP2-97072-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MAGOH Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human MAGOH (Accession#: NP_002361.1; Met1-Ile146)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MAGOH Antibody

Western Blot: MAGOH Antibody [NBP2-97072]

Western Blot: MAGOH Antibody [NBP2-97072]

Western Blot: MAGOH Antibody [NBP2-97072] - Anti-MAGOH rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: MAGOH Antibody [NBP2-97072]

Immunoprecipitation: MAGOH Antibody [NBP2-97072]

Immunoprecipitation: MAGOH Antibody [NBP2-97072] - MAGOH was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 2 ul anti-MAGOH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-MAGOH rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa

Applications for MAGOH Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGOH

Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. (provided by RefSeq)

Alternate Names

MAGOHAMAGOH1, mago-nashi (Drosophila) homolog, proliferation-associated, mago-nashi homolog, proliferation-associated (Drosophila), protein mago nashi homolog

Gene Symbol

MAGOH

Additional MAGOH Products

Product Documents for MAGOH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAGOH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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