MAGP-2/MFAP5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82803

Novus Biologicals, part of Bio-Techne
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NBP1-82803
NBP1-82803-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAGP-2/MFAP5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVNSQRGDDVTQATPETFTEDPNLVNDPATDETVLAVLADIAPSTDDLASLSEKNTTAECWDEKFTCTRLYSVHRPVKQCIHQLCFTSLRRMYIVNKEICSRLVCKEHEAMKDELCRQMAGLPPRRLRRSNYFRLPPCENV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAGP-2/MFAP5 Antibody

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining in human smooth muscle and cerebral cortex tissues using anti-MFAP5 antibody. Corresponding MFAP5 RNA-seq data are presented for the same tissues.
Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803]

Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803]

Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803] - Analysis in human cell line BEWO.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human cerebral cortex shows low expression as expected.
View 3 more images

Applications for MAGP-2/MFAP5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGP-2/MFAP5

MFAP5, also known as Microfibrillar-associated protein 5, is a 173 amino acid protein that is 20 kDa, extracellular matrix secreted, and it is a component of the elastin-associated microfibrils. Current research is being performed on this protein involvement in marfan syndrome, scleroderma and skin fibrosis. The protein is involved in molecules associated with elastic fibres, extracellular matrix organization, elastic fibre formation, tropoelastin associates with microfibrils, elastin cross-linking by lysyl oxidase, and notch signaling pathways where it interacts with FBN2, JAG1, JAG2, MEGF6, VWF, FBLN1, FBN1, GYG1, DLL1, MFAP2, NOTCH1, ENAM, FBN3, and DLL4 proteins.

Long Name

Microfibril-associated Glycoprotein 2

Alternate Names

MAGP2, MFAP5, MP25

Gene Symbol

MFAP5

Additional MAGP-2/MFAP5 Products

Product Documents for MAGP-2/MFAP5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAGP-2/MFAP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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