MALT1 Antibody (PSH02-32)

Western Blot: MALT1 Antibody (PSH02-32) [NBP3-32562]

Western Blot: MALT1 Antibody (PSH02-32) [NBP3-32562] - Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (NBP3-32562) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate
Lane 2: Hela cell lysate
Lane 3: Ramos cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Daudi cell lysate
Lane 8: 786-0 cell lysate
Lane 9: LNCaP cell lysate
Lane 10: PC-3M cell lysate
Lane 11: SW480 cell lysate
Lane 12: Mouse lymph node(no heat)tissue lysate
Lane 13: Mouse thymus tissue lysate
Lane 14: Rat thymus tissue lysate

Cell lysates/proteins at 20 ug/Lane, tissue lysates/proteins at 40 ug/Lane.

Predicted band size: 92 kDa
Observed band size: 92 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32562) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MALT1 Antibody (PSH02-32) [NBP3-32562]

Immunohistochemistry: MALT1 Antibody (PSH02-32) [NBP3-32562] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MALT1 antibody (NBP3-32562) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32562) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: MALT1 Antibody (PSH02-32) [NBP3-32562]

Immunocytochemistry/ Immunofluorescence: MALT1 Antibody (PSH02-32) [NBP3-32562] - Immunocytochemistry analysis of Ramos cells labeling MALT1 with Rabbit anti-MALT1 antibody (NBP3-32562) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MALT1 antibody (NBP3-32562) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.