MAML1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1306

Novus Biologicals, part of Bio-Techne
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NB100-1306
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MAML1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence VLPTCPMAEFALPR corresponding to N-Terminus according to NP_055572.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MAML1 Antibody

Western Blot: MAML1 Antibody [NB100-1306]

Western Blot: MAML1 Antibody [NB100-1306]

Western Blot: MAML1 Antibody [NB100-1306] - (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MAML1 Antibody [NB100-1306]

Immunohistochemistry-Paraffin: MAML1 Antibody [NB100-1306]

Immunohistochemistry-Paraffin: MAML1 Antibody [NB100-1306] - 3.8ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MAML1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human skeletal muscle lysates (calculated MW of 108 kDa band according to NP_055572.1). IHC-P: Human placenta shows nuclear staining in trophoblasts.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAML1

MAML1 is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway.

Alternate Names

FLJ53540, KIAA0200Mam1, Mam-1mastermind homolog, mastermind (drosophila)-like 1, mastermind-like 1 (Drosophila), mastermind-like protein 1

Entrez Gene IDs

9794 (Human)

Gene Symbol

MAML1

UniProt

NP_055572. (Human)

Additional MAML1 Products

Product Documents for MAML1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAML1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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