MANF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32037

Novus Biologicals, part of Bio-Techne
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NBP1-32037
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (99%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MANF Antibodies »

Product Summary for MANF Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human MANF. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (84%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MANF Antibody

Western Blot: MANF Antibody [NBP1-32037]

Western Blot: MANF Antibody [NBP1-32037]

Western Blot: MANF Antibody [NBP1-32037] - ARMET Antibody [NBP1-32037] - Sample(30 ug of whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry/ Immunofluorescence: MANF Antibody [NBP1-32037]

Immunocytochemistry/ Immunofluorescence: MANF Antibody [NBP1-32037]

Immunocytochemistry/Immunofluorescence: ARMET Antibody [NBP1-32037] - Analysis of methanol-fixed HepG2, using antibody at 1:200 dilution.

Applications for MANF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 40% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MANF

This gene encodes a highly conserved protein whose function is not yet known. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon.

Long Name

Mesencephalic Astrocyte-derived Neurotrphic Factor

Alternate Names

ARMET, ARP

Gene Symbol

MANF

Additional MANF Products

Product Documents for MANF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MANF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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