MANF Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76836
![Western Blot: MANF AntibodyBSA Free [NBP1-76836]](https://resources.bio-techne.com/images/products/MANF-Antibody-Western-Blot-NBP1-76836-img0007.jpg "Western Blot: MANF AntibodyBSA Free [NBP1-76836]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (92%). Backed by our 100% Guarantee.
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Summary for MANF Antibody - BSA Free
Immunogen
Antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human MANF. The immunogen is located within the last 50 amino acids of MANF. Amino Acid Squence: KYAPKAASARTD
Specificity
This antibody does not cross-react with CDNF.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MANF Antibody - BSA Free
Western Blot: MANF AntibodyBSA Free [NBP1-76836]
Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76836 (Lane 1: 0.125 ug /mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.![Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76836]](https://resources.bio-techne.com/images/products/MANF-Antibody-Immunohistochemistry-NBP1-76836-img0004.jpg "Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76836]")
Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76836]
Immunohistochemistry: MANF Antibody [NBP1-76836] - Analysis of MANF in human brain tissue with MANF antibody at 2.5 ug/ml.![Western Blot: MANF AntibodyBSA Free [NBP1-76836]](https://resources.bio-techne.com/images/products/MANF-Antibody-Western-Blot-NBP1-76836-img0006.jpg "Western Blot: MANF AntibodyBSA Free [NBP1-76836]")
Western Blot: MANF AntibodyBSA Free [NBP1-76836]
Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76836 (2 ug /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Applications for MANF Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/mL
Western Blot
0.125-2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MANF
Long Name
Mesencephalic Astrocyte-derived Neurotrphic Factor
Alternate Names
ARMET, ARP
Gene Symbol
MANF
UniProt
Additional MANF Products
Product Documents for MANF Antibody - BSA Free
Product Specific Notices for MANF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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