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Mannose Phosphate Isomerase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17215

Novus Biologicals, part of Bio-Techne
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NBP2-17215

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Primate (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Mannose Phosphate Isomerase Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Mannose Phosphate Isomerase. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (91%), Bovine (87%), Mouse (80%), Rat (82%), Pig (86%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mannose Phosphate Isomerase Antibody

Knockout Validated: Mannose Phosphate Isomerase Antibody [NBP2-17215]

Knockout Validated: Mannose Phosphate Isomerase Antibody [NBP2-17215]

Knockout Validated: Mannose Phosphate Isomerase Antibody [NBP2-17215] - Wild-type (WT) and MPI knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MPI antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Mannose Phosphate Isomerase Antibody [NBP2-17215]

Western Blot: Mannose Phosphate Isomerase Antibody [NBP2-17215]

Western Blot: Mannose Phosphate Isomerase Antibody [NBP2-17215] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody [NBP2-17215]

Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody [NBP2-17215]

Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody [NBP2-17215] - Immunohistochemical analysis of paraffin-embedded TOV21G xenograft, using antibody at 1:500 dilution.

Applications for Mannose Phosphate Isomerase Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Mannose Phosphate Isomerase

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq]

Alternate Names

CDG1B, EC 5.3.1, EC 5.3.1.8, FLJ39201, mannose phosphate isomerase, mannose-6- phosphate isomerase, mannose-6-phosphate isomerase, Phosphohexomutase, Phosphomannose isomerase, phosphomannose isomerase 1, PMI, PMI1phosphohexomutase

Gene Symbol

MPI

UniProt

Additional Mannose Phosphate Isomerase Products

Product Documents for Mannose Phosphate Isomerase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mannose Phosphate Isomerase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.