MAP1B [p Thr1265] Antibody - SuperBUGS

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42827

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Fish, Monkey, Reptile, Xenopus

Cited:

Human, Mouse, Rat, Avian - Chicken, Fish, Frog - Xenopus (African Clawed Frog), Monkery, Reptile

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.12 mg/ml

View all MAP1B Antibodies »

Product Specifications

Immunogen

Phosphopeptide made to a C-terminal portion of rat MAP1B. [Swiss-Prot# P15205]

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:22654911).

Modification

p Thr1265

Specificity

Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

320 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP1B [p Thr1265] Antibody - SuperBUGS

Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Analysis of phosphorylated MAP1B in chick embryos (lane 1) and rat neonatal brain (lane 2).
Immunohistochemistry: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Immunohistochemical analysis of rat spinal cord transverse section from an E12 embryo.
Western Blot: MAP1B [p Thr1265] Antibody - SuperBUGS [NBP1-42827] - Western blot analysis of p62, Nbr1, MAP1B-HC, and MAP1B-LC1 protein levels following blockage of autophagic protein degradation. Phospho-MAP1B-HC is degraded by autophagy. Upon blockage of autophagic degradation with Bafilomycin A1: (Baf), levels of phospho-MAP1B-HC increase compared with levels of total MAP1B-HC. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/ijcb/2012/208014/), licensed under a CC-BY license.

Applications for MAP1B [p Thr1265] Antibody - SuperBUGS

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:1000

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000-1:3000
Application Notes
This MAP1B [p Thr1265] antibody (also known as SuperBUGS) is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB a band can be seen at ~320 kDa. Recommended Blocking Buffer for ICC/IF and IHC: 5% (v/v) normal horse serum, 5% (v/v) normal goat serum, 0.2% (w/v) L-lysine, 0.2% (v/v) Triton X-100 in PBS pH 7.2

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

0.12 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP1B

MAP1B (microtubule-associated protein 1B) is the major cytoskeletal constituent of developing nervous system wherein it plays a key role in neuronal migration as well as differentiation. MAP1B interacts with LC1, ANP32A, TIAM2, GAN, DAPK1, tubulin tyrosine TTL etc. and binds at least two tubulin subunits in the polymer which is critical in nucleating microtubule polymerization as well as in stabilizing the microtubules. MAP1B is one of the major phosphorylation substrates for GSK-3beta which phosphorylates it at Ser1260/Thr1265 in differentiating neurons, a molecular switch for MAP1B mediated regulation of microtubule dynamics in the growing axons and the growth cones. MAP1B acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation/membrane blebbing and also brings about tyrosination of alpha-tubulin in neuronal microtubules along with its essential role in synaptic maturation. MAP1B is downstream of various signalling pathways that stimulate neurite growth or direct growth cone pathfinding, e.g. laminin, netrins, neurotrophins, wingless/integration site, or that influence neuronal migration, e.g. reelin. MAP1B is implicated in a number of neurological disorders, such as fragile X syndrome and giant axonal neuropathy.

Alternate Names

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP-1B, MAP5, microtubule-associated protein 1B

Gene Symbol

MAP1B

Additional MAP1B Products

Product Documents for MAP1B [p Thr1265] Antibody - SuperBUGS

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Certificate of Analysis

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Product Specific Notices for MAP1B [p Thr1265] Antibody - SuperBUGS

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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