Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4H5

Concentration

1 mg/ml

View all MAP2 Antibodies »

Product Specifications

Immunogen

MAP2 Antibody (4H5) was developed against full length purified bovine protein, epitope mapped to projection domain of human sequence, between amino acids 631 and 1056.

Localization

Cytoskeleton

Specificity

MAP2 Antibody (4H5) will be reactive to isoforms 1(MAP2B) and isoform 3 (MAP2A).

Marker

Neuronal Dendritic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

199 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP2 Antibody (4H5)

Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156]

Immunocytochemistry/ Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156]

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.
Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156]

Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156]

Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP2-25156 antibody labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.
Western Blot: MAP2 Antibody (4H5) [NBP2-25156]

Western Blot: MAP2 Antibody (4H5) [NBP2-25156]

Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.
View 3 more images

Applications for MAP2 Antibody (4H5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry Free-Floating

1:2000

Immunohistochemistry-Paraffin

1:300

Western Blot

1:10000
Application Notes
This MAP2 (4H5) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~280 kDa. Use in IHC-P reported in verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using NBP2-25156 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP2

Microtubule-associated protein 2 also known as MAP2 (theoretical molecular weight 280 kDa) belongs to the MAP2/Tau family which in addition to the two neuronal forms MAP2 and Tau also includes MAP4 (1). For MAP2, four different isoforms have been identified which are produced through alternative splicing including MAP2a, MAP2b, MAP2c and MAP2d (1). MAP2 isoforms contain multiple microtubule-binding domains located near the carboxy terminal, a variably sized amino-terminal projection domain, and a conserved protein kinase A binding domain. MAP2c and MAP2d isoforms have a shorter amino-terminal projection domain, lower molecular weight and are identifiable at ~70 kDa in SDS-PAGE analysis (2,3). MAP2's carboxy terminal domain supports interactions with microtubules, actin, and intermediate filaments (2).

MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).

References

1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204

2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384

3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642

4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3

Long Name

Microtubule-associated Protein 2

Alternate Names

DKFZp686I2148, MAP-2, MAP2A, MAP2B, MAP2C, Microtubule Associated Protein 2, microtubule-associated protein 2, MTAP2

Entrez Gene IDs

4133 (Human)

Gene Symbol

MAP2

OMIM

602533 (Human)

UniProt

P11137

Additional MAP2 Products

Product Documents for MAP2 Antibody (4H5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAP2 Antibody (4H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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