MAP2 Antibody (HL1656) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25555
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1656
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAP2 Antibody (HL1656) - Azide and BSA Free
Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] -
Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] -
Immunocytochemistry/Immunofluorescence: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] -
Immunohistochemistry-Paraffin: MAP2 Antibody (HL1656) - Azide and BSA Free [NBP3-25555] - MAP2 antibody [HL1656] detects MAP2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. MAP2 stained by MAP2 antibody [HL1656] (NBP3-25555) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for MAP2 Antibody (HL1656) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MAP2
MAP2 isoforms are developmentally regulated and differentially expressed in neurons and some glia. MAP2c is predominantly expressed in the developing brain while the other isoforms are expressed in the adult brain. The distribution of MAP2 isoforms also varies, with MAP2a and MAP2b predominantly localized to dendrites, while MAP2c is also found in axons. Lastly, the expression of MAP2d is not limited to neurons and may be found in glia, specifically oligodendrocytes (1, 2). MAP2 isoforms associate with microtubules and mediate their interaction with actin filaments thereby playing a critical role in organizing the microtubule-actin network. In neurons, MAP2 isoforms are implicated in different processes including neurite initiation, elongation and stabilization as well as axon and dendrite formation (2). Knockout of MAP expression in animal models results in a variety of functional and structural brain defects according to the isoform affected (e.g., reduced LTP and LTD, reduced myelination, absence of corpus collosum, motor system malfunction, abnormal hippocampal dendritic morphology, abnormal synaptic plasticity) (4).
References
1. Dehmelt, L., & Halpain, S. (2005). The MAP2/Tau family of microtubule-associated proteins. Genome Biology. https://doi.org/10.1186/gb-2004-6-1-204
2. Mohan, R., & John, A. (2015). Microtubule-associated proteins as direct crosslinkers of actin filaments and microtubules. IUBMB Life. https://doi.org/10.1002/iub.1384
3. Shafit-Zagardo, B., & Kalcheva, N. (1998). Making sense of the multiple MAP-2 transcripts and their role in the neuron. Molecular Neurobiology. https://doi.org/10.1007/BF02740642
4. Tortosa, E., Kapitein, L. C., & Hoogenraad, C. C. (2016). Microtubule organization and microtubule-associated proteins (MAPs). In Dendrites: Development and Disease. https://doi.org/10.1007/978-4-431-56050-0_3
Long Name
Microtubule-associated Protein 2
Alternate Names
DKFZp686I2148, MAP-2, MAP2A, MAP2B, MAP2C, Microtubule Associated Protein 2, microtubule-associated protein 2, MTAP2
Gene Symbol
MAP2
Additional MAP2 Products
Product Documents for MAP2 Antibody (HL1656) - Azide and BSA Free
Product Specific Notices for MAP2 Antibody (HL1656) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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