MAP3K12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-17218

Novus Biologicals, part of Bio-Techne
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NBP2-17218
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAP3K12 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse MAP3K12. The exact sequence is proprietary.

Localization

Cytoplasm; Membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP3K12 Antibody

Western Blot: MAP3K12 Antibody [NBP2-17218]

Western Blot: MAP3K12 Antibody [NBP2-17218]

Western Blot: MAP3K12 Antibody [NBP2-17218] - A. 20 ug mouse embryonic stem cell whole cell lysate/extract 7.5 % SDS-PAGEDLK/MAP3K12 antibody dilution: 1:1000
Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218]

Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218]

Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218] - Frozen sectioned E13.5 Rat brain. Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: MAP3K12 Antibody [NBP2-17218]

Western Blot: MAP3K12 Antibody [NBP2-17218]

Western Blot: MAP3K12 Antibody [NBP2-17218] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000.
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Applications for MAP3K12 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

Reported by internal validation

Western Blot

1:500-1:3000
Application Notes
In Simple Western internal validation: Mouse Retinal Ganglion Cells as sample; separated by size; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP3K12

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Alternate Names

DLKEC 2.7.11.25, Dual leucine zipper bearing kinase, EC 2.7.11, Leucine-zipper protein kinase, MAPK-upstream kinase, MEKK12, mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase, MUKdual leucine zipper kinase DLK, protein kinase MUK, ZPKleucine zipper protein kinase, ZPKP1

Gene Symbol

MAP3K12

UniProt

Q60700 (Mouse)

Additional MAP3K12 Products

Product Documents for MAP3K12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAP3K12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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