MAP3K6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51977

Novus Biologicals, part of Bio-Techne
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NBP1-51977
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Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MAP3K6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RAILAQRAGSTP corresponding to C-Terminus according to NP_004663.3.

Epitope

C-RAILAQRAGSTP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MAP3K6 Antibody

Western Blot: MAP3K6 Antibody [NBP1-51977]

Western Blot: MAP3K6 Antibody [NBP1-51977]

Western Blot: MAP3K6 Antibody [NBP1-51977] - (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MAP3K6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 140 kDa band observed in rat heart lysates (calculated MW of 143 kDa band according to human NP_004663.3 and rat NP_001101379.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAP3K6

This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined.

Alternate Names

apoptosis signal regulating kinase 2, ASK2MGC20114, EC 2.7.11, EC 2.7.11.25, MAPKKK6MEKK6Apoptosis signal-regulating kinase 2, MGC125653, mitogen-activated protein kinase kinase kinase 6

Entrez Gene IDs

9064 (Human); 313022 (Mouse); 53608 (Rat)

Gene Symbol

MAP3K6

UniProt

NP_004663.3 (Human)

Additional MAP3K6 Products

Product Documents for MAP3K6 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAP3K6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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