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MAP4 Antibody (7C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00004134-M03

Novus Biologicals, part of Bio-Techne
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H00004134-M03

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MAP4 (NP_112147.2, 1 a.a. ~ 99 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADLSLADALTEPSPDIEGEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSETSQIEDTPSSKPTLLANGGHGVEGSDTTEA

Marker

Microtubule Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MAP4 Antibody (7C9)

Western Blot: MAP4 Antibody (7C9) [H00004134-M03]

Western Blot: MAP4 Antibody (7C9) [H00004134-M03]

Western Blot: MAP4 Antibody (7C9) [H00004134-M03] - Analysis of MAP4 expression in transfected 293T cell line by MAP4 monoclonal antibody (M03), clone 7C9. Lane 1: MAP4 transfected lysatE (10.4 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: MAP4 Antibody (7C9) [H00004134-M03] - Detection limit for recombinant GST tagged MAP4 is 0.1 ng/ml as a capture antibody.

Applications for MAP4 Antibody (7C9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP4

MAP4 is encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.

Alternate Names

DKFZp779A1753, MAP-4, MGC8617, microtubule-associated protein 4

Gene Symbol

MAP4

Additional MAP4 Products

Product Documents for MAP4 Antibody (7C9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP4 Antibody (7C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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