MAP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74616

Novus Biologicals, part of Bio-Techne
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NB100-74616
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all MAP4 Antibodies »

Product Summary for MAP4 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1102 and 1152 of human microtubule-associated protein 4 using the numbering given in entry NP_002366.2 (GeneID 4134).

Marker

Microtubule Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP4 Antibody

Western Blot: MAP4 Antibody [NB100-74616]

Western Blot: MAP4 Antibody [NB100-74616]

Western Blot: MAP4 Antibody [NB100-74616] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-74616used for WB at 0.04 mcg/ml (A) and 0.01 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MAP4 was also immunoprecipitated by rabbit anti-MAP4 antibody NB100-74615, which recognizes an upstream epitope.
Immunohistochemistry: MAP4 Antibody [NB100-74616]

Immunohistochemistry: MAP4 Antibody [NB100-74616]

Immunohistochemistry: MAP4 Antibody [NB100-74616] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: MAP4 Antibody [NB100-74616]

Immunohistochemistry-Paraffin: MAP4 Antibody [NB100-74616]

Immunohistochemistry-Paraffin: MAP4 Antibody [NB100-74616] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-MAP4 used at a dilution of 1:100.

Applications for MAP4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MAP4

The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.

Alternate Names

DKFZp779A1753, MAP-4, MGC8617, microtubule-associated protein 4

Entrez Gene IDs

4134 (Human)

Gene Symbol

MAP4

UniProt

P27816 (Human)

Additional MAP4 Products

Product Documents for MAP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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