MAP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89483

Novus Biologicals, part of Bio-Techne
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NBP1-89483
NBP1-89483-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAP4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EAPLAKDGVLTLANNVTPAKDVPPLSETEATPVPIKDMEIAQTQKGISEDSHLESLQDVGQSAAPTFMISPETVTGT

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261).

Marker

Microtubule Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP4 Antibody

Western Blot: MAP4 Antibody [NBP1-89483]

Western Blot: MAP4 Antibody [NBP1-89483]

Western Blot: MAP4 Antibody [NBP1-89483] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: MAP4 Antibody [NBP1-89483]

Immunocytochemistry/ Immunofluorescence: MAP4 Antibody [NBP1-89483]

Immunocytochemistry/Immunofluorescence: MAP4 Antibody [NBP1-89483] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483]

Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483]

Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human kidney.
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Applications for MAP4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP4

MAP4 is encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.

Alternate Names

DKFZp779A1753, MAP-4, MGC8617, microtubule-associated protein 4

Gene Symbol

MAP4

Additional MAP4 Products

Product Documents for MAP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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