MAP4K1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1045

Novus Biologicals, part of Bio-Techne
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NB100-1045
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MAP4K1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence DVVDPDIFNRDPR-C corresponding to N-Terminus according to NP_001036065.1, NP_009112.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Specificity

This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MAP4K1 Antibody

Western Blot: MAP4K1 Antibody [NB100-1045]

Western Blot: MAP4K1 Antibody [NB100-1045]

Western Blot: MAP4K1 Antibody [NB100-1045] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: MAP4K1 Antibody [NB100-1045]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NB100-1045]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NB100-1045] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MAP4K1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 95-100 kDa band observed in Jurkat (predicted MW of 96 kDa band according to NP_009112).
IHC: Paraffin embedded Human Thymus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAP4K1

MAP4K1 or HPKI (hematopoietic progenitor kinase I) is one of these mammalian kinases that have significant sequence similarity to the Saccharomyces Cerevisiae serine/threonine kinase STE20, which relays signals from G protein coupled receptors to cytosolic MAP kinase cascades. MAP4K1 may play a role in the response to environmental stress. It appears to act upstream of the JUN N terminal pathway. It may play a role in hematopoietic lineage decisions and growth regulation.

Alternate Names

EC 2.7.11, EC 2.7.11.1, Hematopoietic progenitor kinase, HPK1hematopoietic progenitor kinase 1, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, mitogen-activated protein kinase kinase kinase kinase 1

Entrez Gene IDs

11184 (Human)

Gene Symbol

MAP4K1

Additional MAP4K1 Products

Product Documents for MAP4K1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MAP4K1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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