MAP4K1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12577

Novus Biologicals, part of Bio-Techne
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NBP3-12577-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MAP4K1 Antibodies »

Product Summary for MAP4K1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human MAP4K1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for MAP4K1 Antibody

Western Blot: MAP4K1 Antibody [NBP3-12577]

Western Blot: MAP4K1 Antibody [NBP3-12577]

Western Blot: MAP4K1 Antibody [NBP3-12577] - Lane A: Daudi Whole Cell LysateLane B: Raji Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:97 kDaObserved band size:97 kDa
Immunoprecipitation: MAP4K1 Antibody [NBP3-12577]

Immunoprecipitation: MAP4K1 Antibody [NBP3-12577]

Immunoprecipitation: MAP4K1 Antibody [NBP3-12577] - Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-MAP4K1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MAP4K1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size :97 kDa

Applications for MAP4K1 Antibody

Application
Recommended Usage

Immunoprecipitation

0.2-1ul/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP4K1

The hematopoietic progenitor kinase 1 (HPK1) is a mammalian hematopoiesis-specific Ste20 homologue belonging to the germinal center (GC) kinase family, which contains at least 20 kinases (1). HPK1 is implicated in a variety of signaling systems, including epidermal growth factor, transforming growth factor-beta, erythropoietin, prostaglandin E2, and T cell receptor and B cell receptor stimulation. HPK1 is also involved in Fas ligation-mediated apoptosis, NF-B activation and a highly specific activator of the JNK/SAP signaling pathway (2-5). Phosphorylation of HPK1 on threonine 165, serine 171 and tyrosine 379 is a prerequisite for its enzymatic activation in response to immunoreceptor stimulation (6).

Alternate Names

EC 2.7.11, EC 2.7.11.1, Hematopoietic progenitor kinase, HPK1hematopoietic progenitor kinase 1, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, mitogen-activated protein kinase kinase kinase kinase 1

Gene Symbol

MAP4K1

Additional MAP4K1 Products

Product Documents for MAP4K1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP4K1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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