MAP4K3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81824

Novus Biologicals, part of Bio-Techne
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NBP1-81824
NBP1-81824-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MAP4K3 Antibodies »

Product Summary for MAP4K3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGNGMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP4K3 Antibody

Immunocytochemistry/ Immunofluorescence: MAP4K3 Antibody [NBP1-81824]

Immunocytochemistry/ Immunofluorescence: MAP4K3 Antibody [NBP1-81824]

Immunocytochemistry/Immunofluorescence: MAP4K3 Antibody [NBP1-81824] - Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP4K3 Antibody [NBP1-81824]

Immunohistochemistry-Paraffin: MAP4K3 Antibody [NBP1-81824]

Immunohistochemistry-Paraffin: MAP4K3 Antibody [NBP1-81824] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for MAP4K3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP4K3

Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.

Alternate Names

EC 2.7.11, EC 2.7.11.1, germinal center kinase-like kinase, Germinal center kinase-related protein kinase, GLKMEKKK 3, MAPK/ERK kinase kinase kinase 3, MAPKKKK3, MEK kinase kinase 3, mitogen-activated protein kinase kinase kinase kinase 3, RAB8IPL1

Gene Symbol

MAP4K3

Additional MAP4K3 Products

Product Documents for MAP4K3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MAP4K3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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