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MAP4K5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31234

Novus Biologicals, part of Bio-Techne
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NBP1-31234

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (94%), Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for MAP4K5 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human KHS. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAP4K5 Antibody

Western Blot: MAP4K5 Antibody [NBP1-31234]

Western Blot: MAP4K5 Antibody [NBP1-31234]

Western Blot: MAP4K5 Antibody [NBP1-31234] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: MAP4K5 Antibody [NBP1-31234]

Immunocytochemistry/ Immunofluorescence: MAP4K5 Antibody [NBP1-31234]

Immunocytochemistry/Immunofluorescence: MAP4K5 Antibody [NBP1-31234] - Analysis of A431, using MAP4K5 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-31234]

Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-31234]

Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-31234] - SAS xenograft, using KHS antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for MAP4K5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP4K5

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Long Name

Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase

Alternate Names

GCKR, KHS1, MAPKKKK5

Entrez Gene IDs

11183 (Human)

Gene Symbol

MAP4K5

UniProt

Additional MAP4K5 Products

Product Documents for MAP4K5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAP4K5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.