MAPKAPK2 Antibody (4H8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15173
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4H8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MAPKAPK2 Antibody (4H8)
Immunogen
A synthesized peptide derived from human MAPKAPK2. UniProt ID (P49137).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAPKAPK2 Antibody (4H8)
Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173]
Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173] - Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 43 KDa. Observed band size: 49 KDa.Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173]
Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173] - IHC image of MAPKAPK2 antibody diluted at 1:100 and staining in paraffin-embedded human heart tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173]
Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173] - IHC image of MAPKAPK2 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for MAPKAPK2 Antibody (4H8)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MAPKAPK2
Long Name
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2
Alternate Names
MK2, Rps6kc1
Gene Symbol
MAPKAPK2
Additional MAPKAPK2 Products
Product Documents for MAPKAPK2 Antibody (4H8)
Product Specific Notices for MAPKAPK2 Antibody (4H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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