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MAPKAPK2 Antibody (4H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15173

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15173

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4H8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MAPKAPK2 Antibody (4H8)

Immunogen

A synthesized peptide derived from human MAPKAPK2. UniProt ID (P49137).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAPKAPK2 Antibody (4H8)

Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173]

Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173]

Western Blot: MAPKAPK2 Antibody (4H8) [NBP3-15173] - Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, THP-1 whole cell lysate. All lanes: MAPKAPK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 43 KDa. Observed band size: 49 KDa.
Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173]

Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173]

Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173] - IHC image of MAPKAPK2 antibody diluted at 1:100 and staining in paraffin-embedded human heart tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173]

Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173]

Immunohistochemistry: MAPKAPK2 Antibody (4H8) [NBP3-15173] - IHC image of MAPKAPK2 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for MAPKAPK2 Antibody (4H8)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MAPKAPK2

MAPKAP Kinase 2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Long Name

Mitogen-Activated Protein Kinase-Activated Protein Kinase 2

Alternate Names

MK2, Rps6kc1

Gene Symbol

MAPKAPK2

Additional MAPKAPK2 Products

Product Documents for MAPKAPK2 Antibody (4H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAPKAPK2 Antibody (4H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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