Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 7H4.2

Format

BSA Free

Concentration

1 mg/ml

View all MAPKAPK2 Antibodies »

Product Specifications

Immunogen

KLH-conjugated synthetic peptide of the C-terminus of the long form of human MAPKAP-K2.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_004759.3;NM_032960.2

Scientific Data Images for MAPKAPK2 Antibody (7H4.2) - BSA Free

Western Blot: MAPKAPK2 Antibody (7H4.2)BSA Free [NBP2-29767]

Western Blot: MAPKAPK2 Antibody (7H4.2)BSA Free [NBP2-29767]

Western Blot: MAPKAPK2 Antibody (7H4.2) [NBP2-29767] - Lysates from Hela were resolved by electrophoresis, transferred to PVDF and probed with anti-MAPKAPK2 (1 ug/mL). Proteins were visualized probed with using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates phosphorylated MAPKAPK2 (45 kDa).

Applications for MAPKAPK2 Antibody (7H4.2) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:20

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 mg antibody with PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAPKAPK2

MAPKAP Kinase 2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Long Name

Mitogen-Activated Protein Kinase-Activated Protein Kinase 2

Alternate Names

MK2, Rps6kc1

Gene Symbol

MAPKAPK2

UniProt

P49137 (Human)

Additional MAPKAPK2 Products

Product Documents for MAPKAPK2 Antibody (7H4.2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MAPKAPK2 Antibody (7H4.2) - BSA Free

Relevant Entrez Gene Numbers: NM_004759.3;NM_032960.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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