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MARCH6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93441

Novus Biologicals, part of Bio-Techne
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NB100-93441

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QGRRVIFQKVKE corresponding to internal region according to NP_005876.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MARCH6 Antibody

Western Blot: MARCH6 Antibody [NB100-93441]

Western Blot: MARCH6 Antibody [NB100-93441]

Western Blot: MARCH6 Antibody [NB100-93441] - Staining of Human Lung lysate with antibody at 0.2 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MARCH6 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 - 0.5 ug/mL
Application Notes
WB: Approx. 100 kDa band observed in human lung lysates (calculated MW of 103 kDa band according to NP_005876.2). An additional band of unknown identity was also consistently observed at 18 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MARCH6

MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.

Alternate Names

E3 ubiquitin-protein ligase MARCH6, EC 6.3.2.-, MARCH-VIDOA10, membrane-associated ring finger (C3HC4) 6, Membrane-associated RING finger protein 6, Membrane-associated RING-CH protein VI, Protein TEB-4, RNF176KIAA0597Doa10 homolog, TEB4RING finger protein 176

Entrez Gene IDs

10299 (Human); 223455 (Mouse)

Gene Symbol

MARCHF6

UniProt

Additional MARCH6 Products

Product Documents for MARCH6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MARCH6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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