MARCH8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76959

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all MARCH8 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8. The immunogen is located within the last 50 amino acids of MARCH8. Amino Acid Squence: CPETSKKNIFEKSPLTE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MARCH8 Antibody - BSA Free

Western Blot: MARCH8 AntibodyBSA Free [NBP1-76959]

Western Blot: MARCH8 AntibodyBSA Free [NBP1-76959]

Western Blot: MARCH8 Antibody [NBP1-76959] - HeLa cell lysate with MARCH8 antibody at (A) 0.5 ug/ml and (B) 1 ug/mL.

Immunocytochemistry/Immunofluorescence: MARCH8 Antibody [NBP1-76959] -

Immunocytochemistry/Immunofluorescence: MARCH8 Antibody [NBP1-76959] - HeLa cells with MARCH8 antibody at 2.5 ug/ml.
MARCH8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MARCH8 Antibody - BSA Free [NBP1-76959] -

Immunocytochemistry/ Immunofluorescence: MARCH8 Antibody - BSA Free [NBP1-76959] - Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 antibody at 2.5 u/mL.
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Applications for MARCH8 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5 ug/mL

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MARCH8

MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Long Name

Membrane Associated Ring Finger (C3HC4) 8

Alternate Names

c-MIR, MARCH-VIII, RNF178

Gene Symbol

MARCHF8

UniProt

NP_659458 (Human)

Additional MARCH8 Products

Product Documents for MARCH8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MARCH8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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