MARCKS Antibody (5F9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05539

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5F9

Concentration

1 mg/ml

View all MARCKS Antibodies »

Product Specifications

Immunogen

Full length human MARCKS expressed in and purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MARCKS Antibody (5F9)

Western Blot: MARCKS Antibody [NBP3-05539]

Western Blot: MARCKS Antibody [NBP3-05539]

Western Blot: MARCKS Antibody [NBP3-05539] - Western blot analysis of different cell lysates using MARCKS Antibody in green: [1] protein standard, [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6 and [7] COS1 cells. The band at about 80kDa corresponds to the MARCKS protein detected only in the human and monkey cells. The antibody does not recognize the rodent form of the MARCKS protein.
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05539]

Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP3-05539]

Immunocytochemistry/Immunofluorescence: MARCKS Antibody [NBP3-05539] - Immunofluorescent analysis of HeLa cell culture stained with MARCKS Antibody at a dilution of 1:1,000 in red, and costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. MARCKS antibody binds to MARCKS protein expressed in the membrane structures and in cytoplasm, while the vimentin antibody stains the intermediate filaments.

Applications for MARCKS Antibody (5F9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:2000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: MARCKS

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Long Name

Myristoylated Alanine-rich Protein Kinase C Substrate

Alternate Names

80K-L, MACS, PRKCSL

Gene Symbol

MARCKS

Additional MARCKS Products

Product Documents for MARCKS Antibody (5F9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MARCKS Antibody (5F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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