Mark3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30800

Novus Biologicals, part of Bio-Techne
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NBP3-30800-250ug
NBP3-30800-50ug
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Mark3 Antibodies »

Product Specifications

Immunogen

Recombinant, full-length His-tagged human Mark3 produced in E. Coli.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mark3 Antibody

Mark3 Antibody

Western Blot: Mark3 Antibody [NBP3-30800] -

Western Blot: Mark3 Antibody [NBP3-30800] - Western blot analysis using c-tak1 antibody (Cat. No. NBP3-30800) on His-tagged c-tak1 (1), RKO cell lysate (2) an HCT116 cell lysate (3).

Applications for Mark3 Antibody

Application
Recommended Usage

Western Blot

: 1-5 ug/ml
Application Notes
Western blot: Detects c-tak1 in RKO and HCT116 cell lysates..

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

Phosphate buffered saline

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mark3

MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. MARK3 is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to MARK3, and MARK3/14-3-3 complexes are present throughout interphase but not during mitosis (1). Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (2). MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signalling, indicating a link between two important developmental pathways (3).

Alternate Names

Cdc25C-associated protein kinase 1, cTAK1, C-TAK1, CTAK1ELKL motif kinase 2, EC 2.7.11, EC 2.7.11.1, EMK2, EMK-2, KP78, MAP/microtubule affinity-regulating kinase 3, PAR1A, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78

Gene Symbol

MARK3

Additional Mark3 Products

Product Documents for Mark3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mark3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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