Mas Antibody - BSA Free

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] -

(a) Representative immunolocalisation images of AT1R, AT2R, MasR, and ACE2 in adult WKY rats and aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, and (e) ACE2 expressed as relative fluorescent units in adult (n = 5) and aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats.

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] -

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - (a) Representative immunolocalisation images of AT1R, AT2R, MasR, & ACE2 in adult WKY rats & aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, & (e) ACE2 expressed as relative fluorescent units in adult (n = 5) & aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22187625), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] -

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - Immunofluorescence studies in HEK293T cells overexpressing c-myc tagged MasR using MasR antibodies.Images of fluorescence signal corresponding to secondary cyanine (Cy3)-conjugated antibody (A), nuclear Hoechst 33258 staining (B), merged images (C) & bright field (D) are shown. In cells transfected with the pcDNA 3.1/c-myc-MasR construct, antibodies NLS-1531 & sc-54682 generated a similar staining pattern to that generated with the anti c-myc antibody. No staining was observed with any of these antibodies in cells transfected with the empty vector pcDNA 3.1. Antibody sc-135063 was capable of staining the plasma membrane of cells overexpressing the MasR but also generated widespread signals in cells transfected with the empty vector. For antibody AAR-013 there was no staining associated with the cell membrane. Intense immunocytochemical staining of identical distribution & intensity was revealed in cells transfected with the empty vector & those transfected with the c-myc-MasR construct. Images are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] -

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] - Immunohistochemistry in heart & kidney from wild-type (WT) & MasR-KO mice.Negative controls performed by omitting the primary antibody demonstrated minimal background immunostaining in heart (A) & kidney (B) sections. (A) In heart sections, NLS-1531 antibody stained predominantly the cytoplasm of cardiomyocytes with similar intensity in WT & MasR-KO hearts. The AAR-013 antibody stained the cardiomyocytes nucleus & weaker staining was observed in their cytoplasm. The same pattern was found in heart sections of MasR-KO mice. (B) In kidney sections, for NLS-1531 antibody, staining was mostly restricted to cytoplasm of numerous tubules cells with similar intensity in WT & MasR-KO kidneys. Antibody AAR-013 stained most intensely tubules cells nucleus & weaker staining was observed in their cytoplasm. Glomeruli were also stained positively. The same pattern was found in kidney sections of MasR-KO. Preincubation of the AAR-013 antibody with the blocking peptide provided by the vendors eliminated the immunohistochemical nuclear staining both in WT & MasR-KO mice kidney sections. Images are representative of n = 3. Bar, 50 μm. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.