Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A5

Concentration

1 mg/ml

View all MAST205 Antibodies »

Product Summary for MAST205 Antibody (OTI3A5)

Immunogen

Human recombinant protein fragment corresponding to amino acids 1482-1798 of human MAST2(NP_055927) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

196.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAST205 Antibody (OTI3A5)

Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756]

Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756]

Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756]

Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756]

Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2.

Applications for MAST205 Antibody (OTI3A5)

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAST205

MAST2 (microtubule-associated serine/threonine-protein kinase 2) is also known as MAST205. MAST2 contains a well conserved protein kinase C/A kinase domain and a PDZ domain. It is highly expressed in spermatids and has been shown to play a role in cytokine responses in macrophages.

Alternate Names

EC 2.7.11, EC 2.7.11.1, FLJ39200, KIAA0807RP4-533D7.1, MAST205microtubule associated testis specific serine/threonine protein kinase, microtubule associated serine/threonine kinase 2, microtubule-associated serine/threonine-protein kinase 2, MTSSK

Gene Symbol

MAST2

Additional MAST205 Products

Product Documents for MAST205 Antibody (OTI3A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MAST205 Antibody (OTI3A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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