Max Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-793
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Predicted:
Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EEPQSRKKLRMEAS corresponding to C-Terminus according to NP_002373.3, NP_660087.1.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 24676840).
Specificity
This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Max Antibody
Western Blot: Max Antibody [NB100-793]
Western Blot: Max Antibody [NB100-793] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Max Antibody [NB100-793]
Immunohistochemistry-Paraffin: Max Antibody [NB100-793] - Staining of Prostate. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence: Max Antibody [NB100-793]
Immunofluorescence: Max Antibody [NB100-793] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for Max Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
0.01 - 0.03 ug/mL
Application Notes
WB: Approx 22 kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3 kDa according to NP_002373.3).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Max
Long Name
Myc associated factor X
Alternate Names
BHLHD4, bHLHd4MGC11225, bHLHd5, bHLHd6, bHLHd7, bHLHd8, Class D basic helix-loop-helix protein 4, helix-loop-helix zipper protein, MAX protein, MGC10775, MGC18164, MGC34679, MGC36767, MYC associated factor X, Myc-associated factor X, orf1, protein max
Gene Symbol
MAX
UniProt
Additional Max Products
Product Documents for Max Antibody
Product Specific Notices for Max Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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