MBD1 Antibody (PSH01-78)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32572
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-78
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within mouse MBD1 aa 1-200 / 636. (Uniprot: Q9UIS9)
Localization
Nucleus, Nucleus matrix, Nucleus speckle, Chromosome
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MBD1 Antibody (PSH01-78)
Western Blot: MBD1 Antibody (PSH01-78) [NBP3-32572]
Western Blot: MBD1 Antibody (PSH01-78) [NBP3-32572] - Western blot analysis of MBD1 on different lysates with Rabbit anti-MBD1 antibody (NBP3-32572) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: Human kidney tissue lysate
Lane 6: Mouse kidney tissue lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Mouse lung tissue lysate
Lane 10: Rat brain tissue lysate
Lane 11: Rat testis tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32572) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for MBD1 Antibody (PSH01-78)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MBD1
Alternate Names
CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription
Gene Symbol
MBD1
Additional MBD1 Products
Product Documents for MBD1 Antibody (PSH01-78)
Product Specific Notices for MBD1 Antibody (PSH01-78)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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