MBNL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85635

Novus Biologicals, part of Bio-Techne
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NBP1-85635
NBP1-85635-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MBNL3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AQMSSLGSFPMTPSIPANPPMAFNPYIPHPGMGLVPAELVPNTPVLIPGNPPLAMPGAVGPKLMRSDKLEVCREFQRGNCTRGENDCRYAHPTDASMIEASDNTVTICMDYIKGRCSREKCKYFHPPAHLQARLKAAHHQMNHSAAS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBNL3 Antibody

Immunohistochemistry-Paraffin: MBNL3 Antibody [NBP1-85635]

Immunohistochemistry-Paraffin: MBNL3 Antibody [NBP1-85635]

Immunohistochemistry-Paraffin: MBNL3 Antibody [NBP1-85635] - Staining in human placenta and prostate tissues using anti-MBNL3 antibody. Corresponding MBNL3 RNA-seq data are presented for the same tissues.
Western Blot: MBNL3 Antibody [NBP1-85635]

Western Blot: MBNL3 Antibody [NBP1-85635]

Western Blot: MBNL3 Antibody [NBP1-85635] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Hep-G2
Immunocytochemistry/ Immunofluorescence: MBNL3 Antibody [NBP1-85635]

Immunocytochemistry/ Immunofluorescence: MBNL3 Antibody [NBP1-85635]

Immunocytochemistry/Immunofluorescence: MBNL3 Antibody [NBP1-85635] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
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Applications for MBNL3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBNL3

The MBNL3 gene encodes a member of the muscleblind-like family of proteins. The encoded protein may function in regulation of alternative splicing and may play a role in the pathophysiology of myotonic dystrophy. Alternatively spliced transcript variants have

Alternate Names

CHCRMBLX39FLJ11316, Cys3His CCG1-required protein, FLJ97142, MBXLMBLX, muscleblind-like 3 (Drosophila), muscleblind-like protein 3, Muscleblind-like X-linked protein, Protein HCHCR

Gene Symbol

MBNL3

Additional MBNL3 Products

Product Documents for MBNL3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MBNL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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