Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7G7

Concentration

1 mg/ml

View all MBP Antibodies »

Product Specifications

Immunogen

Purified myelin basic protein isolated from bovine brain, epitope is in peptide TPPPSQGKG, amino acids 125-133 of the human 21.5kDa sequence.

Reactivity Notes

This peptide is invariant in rat, mouse, cow and many other species, so this antibody will have wide applicability. TMW ranges are 14 - 21.5 kDa (rodent) and 17.2 - 21.5 kDa (human).

Specificity

The MBP Antibody (7G7) antibody binds all four of the CNS MBP isoforms, so that the epitope for the antibody is located in the core shared by all four gene products.

Marker

Oligodendrocyte Marker, Myelin Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MBP Antibody (7G7)

Western Blot: MBP Antibody (7G7) [NBP1-05203]

Western Blot: MBP Antibody (7G7) [NBP1-05203]

Western Blot: MBP Antibody (7G7) [NBP1-05203] - Analysis of different tissue lysates using mouse mAb to MBP, NBP1-05203, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.
Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203]

Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203]

Immunohistochemistry: MBP Antibody (7G7) [NBP1-05203] - Analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), NBP1-05203, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H, dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.

Applications for MBP Antibody (7G7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:5000-1:10000
Application Notes
Use in Immunoprecipitation reported in scientific literature (PMID:34880248).This Myelin Basic Protein (7G7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In WB bands can be seen at ~18 and 21.5 kDa. Use in IHC-Fr was reported in scientific literature (PMID: 26482371)..

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic protein (MBP), along with myelin proteolipid protein (PLP/lipophilin), is the most abundant protein constituents of myelin membrane in the CNS cells. MBP plays a key role in both the formation as well as the stabilization of this compact multilayer arrangement of bilayers. MBP is a homodimer that can self-associates in the presence of lysolipid, and is localized in myelin membrane as peripheral membrane protein on the cytoplasmic side of myelin. MBP is found in CNS/PNS and after post-translational modifications (PTMs), at least 6 charge isomers are produced: C1 (most cationic/least modified form), C2, C3, C4, C5 and C6 (the least cationic form); and its potential PTMs includes - phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. MBP phosphorylations are executed by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Citrullination and methylation modifications of MBP may lead to reduction in surface charge that follows its weakened attachment with myelin membranes and this mechanism has been proposed to be operative in demyelinating diseases such as chronical multiple sclerosis (MS), fulminating MS (Marburg disease) etc.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

24547 (Rat)

Gene Symbol

MBP

Additional MBP Products

Product Documents for MBP Antibody (7G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MBP Antibody (7G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.