MBP Antibody (CL2819)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46630

Novus Biologicals, part of Bio-Techne
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NBP2-46630
NBP2-46630-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL2819

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MBP Antibodies »

Product Specifications

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence DENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPM

Epitope

RTPPPSQGKG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MBP Antibody (CL2819)

Western Blot: MBP Antibody (CL2819) [NBP2-46630]

Western Blot: MBP Antibody (CL2819) [NBP2-46630]

Western Blot: MBP Antibody (CL2819) [NBP2-46630] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630]

Immunocytochemistry/ Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630]

Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2819) [NBP2-46630] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630]

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630]

Immunohistochemistry: MBP Antibody (CL2819) [NBP2-46630] - Staining of human tonsil shows absence of immunoreactivity (negative control).
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Applications for MBP Antibody (CL2819)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human)

Gene Symbol

MBP

UniProt

P02686

Additional MBP Products

Product Documents for MBP Antibody (CL2819)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MBP Antibody (CL2819)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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