MBP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-79873
![Western Blot: MBP AntibodyBSA Free [NB110-79873]](https://resources.bio-techne.com/images/products/MBP-Antibody-Western-Blot-NB110-79873-img0001.jpg "Western Blot: MBP AntibodyBSA Free [NB110-79873]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
ELISA, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Reacts with residues 49-62 of Isoforms 5 and 6 of the human protein. The immunogen corresponds to Isoform 1 between amino acids 182-195 of the 33 kDa isoform. The antibody also detects mouse and rat MBP protein. The antibody primarily detects a 22 kDa band.
Reactivity Notes
Human, mouse and rat.
Specificity
Myelin Basic Protein, Reacts with residues 49-62 of the 33 kDa human, mouse and rat MBP protein. Antibody detects primarily the 22 kDa isoform.
Marker
Oligodendrocyte Marker, Myelin Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MBP Antibody - BSA Free
Western Blot: MBP AntibodyBSA Free [NB110-79873]
Western Blot: MBP Antibody [NB110-79873] - WB analysis of Myelin Basic Protein (MBP) in 10ug of normal brain lysate with antibody NB110-79873 at 1:500 dilution.
Simple Western: MBP Antibody [NB110-79873] -
Simple Western: MBP Antibody [NB110-79873] - Simple Western lane view shows a specific band for MBP in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image generated by internal validation.Applications for MBP Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:200
Simple Western
reported by internal validation
Western Blot
1:500-1:1000
Application Notes
This antibody is useful in ELISA, Immunocytochemistry, Immunohistochemistry 1:100-1:200 and Western Blot. In WB a band is seen at approx. 22 kDa representing MBP. Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26616873).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MBP
Long Name
Myelin Basic Protein
Alternate Names
Hmbpr, mld, shi
Gene Symbol
MBP
UniProt
Additional MBP Products
Product Documents for MBP Antibody - BSA Free
Product Specific Notices for MBP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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