MBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29656

Novus Biologicals, part of Bio-Techne
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NBP2-29656
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MBP Antibodies »

Product Summary for MBP Antibody

Immunogen

Three peptide sequences conserved in higher vertebrate MBP proteins. Accession # P02688

Reactivity Notes

It is expected that the antibody will react in other mammalian tissues.

Specificity

Specific for endogenous levels of the ~18 kDa & ~22 kDa myelin basic protein.

Marker

Oligodendrocyte Marker, Myelin Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for MBP Antibody

Western Blot: MBP Antibody [NBP2-29656]

Western Blot: MBP Antibody [NBP2-29656]

Western Blot: MBP Antibody [NBP2-29656] - rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22k MBP protein.
Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP2-29656]

Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP2-29656]

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.
Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP2-29656]

Immunocytochemistry/ Immunofluorescence: MBP Antibody [NBP2-29656]

Immunocytochemistry/Immunofluorescence: MBP Antibody [NBP2-29656] - Rat mixed neuron/glial cultures stained with chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
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Applications for MBP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:500 - 1:5000

Western Blot

1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

PBS

Preservative

0.07% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

24547 (Rat)

Gene Symbol

MBP

UniProt

P02688 (Rat)

Additional MBP Products

Product Documents for MBP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MBP Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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