MBP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50035
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified myelin basic protein isolated from bovine brain. [UniProt# P02687]
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Scientific Data Images for MBP Antibody
![Western Blot: MBP Antibody [NBP2-50035]](https://resources.bio-techne.com/images/products/MBP-Antibody-NBP2-50035-img0002.jpg "Western Blot: MBP Antibody [NBP2-50035]")
Western Blot: MBP Antibody [NBP2-50035]
Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.![Immunohistochemistry: MBP Antibody [NBP2-50035]](https://resources.bio-techne.com/images/products/MBP-Antibody-NBP2-50035-img0001.jpg "Immunohistochemistry: MBP Antibody [NBP2-50035]")
Immunohistochemistry: MBP Antibody [NBP2-50035]
Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.Applications for MBP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000
Immunohistochemistry
1:5000
Western Blot
1:10000
Application Notes
In Western blot, this antibody recognizes all four CNS isotypes of rat MBP with bands at 21.5 kDa, 18.5kDa, 17kDa and 14kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgY purified
Formulation
PBS. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
Preservative
0.035% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MBP
Long Name
Myelin Basic Protein
Alternate Names
Hmbpr, mld, shi
Gene Symbol
MBP
Additional MBP Products
Product Documents for MBP Antibody
Product Specific Notices for MBP Antibody
Chicken products cannot be exported to Canada.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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