MBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50035

Novus Biologicals, part of Bio-Techne
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NBP2-50035
NBP2-50035SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MBP Antibodies »

Product Specifications

Immunogen

Purified myelin basic protein isolated from bovine brain. [UniProt# P02687]

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for MBP Antibody

Western Blot: MBP Antibody [NBP2-50035]

Western Blot: MBP Antibody [NBP2-50035]

Western Blot: MBP Antibody [NBP2-50035] - Analysis of different tissue lysates using chicken pAb to MBP, NBP2-50035, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, and [6] mouse spinal cord. Multiple bands between 14-21.5kDa correspond to various alternate transcripts of the single MBP gene.
Immunohistochemistry: MBP Antibody [NBP2-50035]

Immunohistochemistry: MBP Antibody [NBP2-50035]

Immunohistochemistry: MBP Antibody [NBP2-50035] - Analysis of rat brain cerebellum section stained with chicken pAb to Myelin Basic Protein (MBP), NBP2-50035, dilution 1:5,000 in green, and costained with rabbit pAb to NF-L, NBP1-05218, dilution 1:5,000 in red. The MBP antibody stains oligodendrocytes and myelin sheathes around axons. NBP1-05218 antibody labels dendrites and axons of neuronal cells.

Applications for MBP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:5000

Western Blot

1:10000
Application Notes
In Western blot, this antibody recognizes all four CNS isotypes of rat MBP with bands at 21.5 kDa, 18.5kDa, 17kDa and 14kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

PBS. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.

Preservative

0.035% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Gene Symbol

MBP

Additional MBP Products

Product Documents for MBP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MBP Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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