MCAF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97607

Novus Biologicals, part of Bio-Techne
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NBP2-97607-100ul
NBP2-97607-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MCAF1 Antibodies »

Product Summary for MCAF1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human MCAF1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MCAF1 Antibody

Immunocytochemistry/ Immunofluorescence: MCAF1 Antibody [NBP2-97607]

Immunocytochemistry/ Immunofluorescence: MCAF1 Antibody [NBP2-97607]

Immunocytochemistry/Immunofluorescence: MCAF1 Antibody [NBP2-97607] - Immunofluorescence staining of MCAF1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MCAF1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Applications for MCAF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCAF1

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Alternate Names

activating transcription factor 7 interacting protein, activating transcription factor 7-interacting protein 1, ATF7-interacting protein, ATFa-associated modulator, ATF-interacting protein, ATF-IP, FLJ10139, FLJ10688, hAM, MBD1-containing chromatin associated factor, MCAF1AM, MCAFMBD1-containing chromatin-associated factor 1, MMCP 6, MMCP6, MMCP-6, p621

Gene Symbol

ATF7IP

Additional MCAF1 Products

Product Documents for MCAF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCAF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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