MCC Antibody (1A7)
Novus Biologicals, part of Bio-Techne | Catalog # H00004163-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1A7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MCC (AAH09279, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDVQERTTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRS
Specificity
MCC - mutated in colorectal cancers
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for MCC Antibody (1A7)
![Western Blot: MCC Antibody (1A7) [H00004163-M01]](https://resources.bio-techne.com/images/products/MCC-Antibody-1A7-Western-Blot-H00004163-M01-img0006.jpg "Western Blot: MCC Antibody (1A7) [H00004163-M01]")
Western Blot: MCC Antibody (1A7) [H00004163-M01]
Western Blot: MCC Antibody (1A7) [H00004163-M01] - Analysis of MCC expression in transfected 293T cell line by MCC monoclonal antibody (M01), clone 1A7.Lane 1: MCC transfected lysate(93.1 KDa).Lane 2: Non-transfected lysate.![Western Blot: MCC Antibody (1A7) [H00004163-M01]](https://resources.bio-techne.com/images/products/MCC-Antibody-1A7-Western-Blot-H00004163-M01-img0004.jpg "Western Blot: MCC Antibody (1A7) [H00004163-M01]")
Western Blot: MCC Antibody (1A7) [H00004163-M01]
Western Blot: MCC Antibody (1A7) [H00004163-M01] - Analysis of MCC over-expressed 293 cell line, cotransfected with MCC Validated Chimera RNAi ( Cat # H00004163-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCC monoclonal antibody (M01), clone 1A7 (Cat # H00004163-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: MCC Antibody (1A7) [H00004163-M01] - Detection limit for recombinant GST tagged MCC is approximately 0.03ng/ml as a capture antibody.
Applications for MCC Antibody (1A7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MCC
Alternate Names
colorectal mutant cancer protein, DKFZp762O1615, FLJ38893, FLJ46755, MCC1, mutated in colorectal cancers, Protein MCC
Entrez Gene IDs
4163 (Human)
Gene Symbol
MCC
OMIM
159350 (Human)
UniProt
Additional MCC Products
Product Documents for MCC Antibody (1A7)
Product Specific Notices for MCC Antibody (1A7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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