mCherry Antibody (HL1482) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25559
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1482
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length mCherry recombinant protein
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for mCherry Antibody (HL1482) - Azide and BSA Free
Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] -
Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] -
Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] -
Immunohistochemistry-Paraffin: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - mCherry antibody [HL1482] detects mCherry protein at cytoplasm by immunohistochemical analysis. Sample:Paraffin-embedded mock and mCherry tagged protein transfected 293T cell pellet. Green: mCherry stained by mCherry antibody [HL1482] (NBP3-25559) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for mCherry Antibody (HL1482) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: mCherry
mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).
References
1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819
2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83
3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992
4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026
5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116
Long Name
mCherry
Alternate Names
DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein
Additional mCherry Products
Product Documents for mCherry Antibody (HL1482) - Azide and BSA Free
Product Specific Notices for mCherry Antibody (HL1482) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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