mCherry Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05558

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat

Concentration

1 mg/ml

View all mCherry Antibodies »

Product Specifications

Immunogen

This mCherry Antibody was developed against full length recombinant mCherry protein

Clonality

Polyclonal

Host

Goat

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mCherry Antibody

Western Blot: mCherry Antibody [NBP3-05558]

Western Blot: mCherry Antibody [NBP3-05558]

Western Blot: mCherry Antibody [NBP3-05558] - Western blot analysis of HEK293 cell lysates using mCherry Antibody at a dilution of 1:2,000, in green [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. Major band at about 30kDa corresponds to mCherry protein.
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP3-05558]

Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP3-05558]

Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP3-05558] - Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with mCherry Antibody at a dilution of 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.

Applications for mCherry Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: mCherry

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy (1). mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone) (2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).

mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).

References

1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819

2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83

3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992

4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026

5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116

Long Name

mCherry

Alternate Names

DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

Additional mCherry Products

Product Documents for mCherry Antibody

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Certificate of Analysis

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Product Specific Notices for mCherry Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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