MCM2 Antibody (0Q10S9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16066
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0Q10S9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MCM2 (P49736). MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MCM2 Antibody (0Q10S9)
Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066]
Western Blot: MCM2 Antibody (0Q10S9) [NBP3-16066] - Western blot analysis of extracts of various cell lines, using MCM2 antibody (NBP3-16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.3s.Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066]
Immunocytochemistry/Immunofluorescence: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunofluorescence analysis of U2OS cells using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066]
Immunohistochemistry-Paraffin: MCM2 Antibody (0Q10S9) [NBP3-16066] - Immunohistochemistry of paraffin-embedded rat spleen using MCM2 Rabbit mAb (NBP3-16066) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for MCM2 Antibody (0Q10S9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MCM2
Long Name
Minichromosome Maintenance Protein 2
Alternate Names
BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin
Gene Symbol
MCM2
Additional MCM2 Products
Product Documents for MCM2 Antibody (0Q10S9)
Product Specific Notices for MCM2 Antibody (0Q10S9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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