MCM2 Antibody (S02-0D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19887

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19887-100ul
NBP3-19887-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-0D3

Concentration

0.3 mg/ml

View all MCM2 Antibodies »

Product Summary for MCM2 Antibody (S02-0D3)

Immunogen

A synthetic peptide of human MCM2 (Uniprot # P49736)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MCM2 Antibody (S02-0D3)

Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887]

Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887]

Western Blot: MCM2 Antibody (S02-0D3) [NBP3-19887] - Western blot detection of MCM2 in Hela, A549, HL-60, U2OS cell lysates using NBP3-19887 (1:1000 diluted). Predicted band size: 102kDa. Observed band size: 125kDa.
Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887]

Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887]

Immunocytochemistry/Immunofluorescence: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunocytochemistry of MCM2 (green) in hela using NBP3-19887 at dilution 1/5, and DAPI (blue)
Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887]

Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887]

Immunohistochemistry-Paraffin: MCM2 Antibody (S02-0D3) [NBP3-19887] - Immunohistochemistry of MCM2 in paraffin-embedded Human tonsil using NBP3-19887 at dilution 1/50

Applications for MCM2 Antibody (S02-0D3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Immunohistochemistry

1/20

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM2

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 Antibody (S02-0D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MCM2 Antibody (S02-0D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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