Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MCM2 Antibodies »

Product Specifications

Immunogen

Immunogen was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 108 according to the numbering given in entry NP_0045117.2 (GeneID 4171).

Modification

p Ser108

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM2 [p Ser108] Antibody

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with EPE (100 uM, 2h). Antibodies: MCM2 was immunoprecipitated using rabbit anti-MCM2 antibody was used for WB at 1 mcg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody . Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).
Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639]

Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639]

Immunohistochemistry-Paraffin: MCM2 [p Ser108] Antibody [NB100-639] - Human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- phospho MCM2 (S108) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639]

Western Blot: MCM2 [p Ser108] Antibody [NB100-639] - Detection of Human phospho MCM2 (Ser108) by Western Blot. Samples: Whole cell lysate from HeLa cells that were untreated or exposed to 8 Gy IR for 1 h or 50 J/m2 for 2 hours. Antibody: Affinity purified rabbit anti-phospho MCM2 (Ser108) antibody (Cat. No. NB100-639) used at 0.2 mcg/ml. Subsequently, total MCM2 was detected to verify loading. Detection: Chemiluminescence with an exposure time of 5 seconds.
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Applications for MCM2 [p Ser108] Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000- 1:5000

Immunohistochemistry-Paraffin

1:1000- 1:5000

Western Blot

1:5000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM2

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML)

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Entrez Gene IDs

4171 (Human)

Gene Symbol

MCM2

Additional MCM2 Products

Product Documents for MCM2 [p Ser108] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCM2 [p Ser108] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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