MCM3 Antibody (3E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00004172-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MCM3 Antibodies »

Product Specifications

Immunogen

MCM3 (NP_002379, 699 a.a. ~ 808 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI

Specificity

MCM3 - MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) (3E1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MCM3 Antibody (3E1)

Western Blot: MCM3 Antibody (3E1) [H00004172-M03]

Western Blot: MCM3 Antibody (3E1) [H00004172-M03]

Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa [L013V1].
Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (3E1) [H00004172-M03]

Immunocytochemistry/ Immunofluorescence: MCM3 Antibody (3E1) [H00004172-M03]

Immunocytochemistry/Immunofluorescence: MCM3 Antibody (3E1) [H00004172-M03] - Analysis of monoclonal antibody to MCM3 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: MCM3 Antibody (3E1) [H00004172-M03]

Western Blot: MCM3 Antibody (3E1) [H00004172-M03]

Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - Analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).
View 1 more images

Applications for MCM3 Antibody (3E1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Sandwich ELISA

1:100-1:2000
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Entrez Gene IDs

4172 (Human)

Gene Symbol

MCM3

UniProt

NP_002379 (Human)

Additional MCM3 Products

Product Documents for MCM3 Antibody (3E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MCM3 Antibody (3E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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