Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MCM3 Antibodies »

Product Specifications

Immunogen

A 38-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11 (LocusLink ID 4172).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM3 Antibody

Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: MCM3 Antibody [NB100-289]

Immunohistochemistry: MCM3 Antibody [NB100-289]

Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.

Applications for MCM3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:5000

Immunohistochemistry-Paraffin

1:1000 to 1:5000

Immunoprecipitation

2-5 ug//mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot and immunoprecipitation. Suggested working dilutions: Western Blot - 1: 2,000-1:10,000 Immunohistochemistry/Immunocytochemistry - ND Immunoprecipitation - 2 to 5 ug/mg lysate** *The investigator should determine the optimal working dilution for a specific application. **Use NB 100-288 (MCM2 antibody) to IP the MCM complex
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM3

MCM3 is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Entrez Gene IDs

4172 (Human)

Gene Symbol

MCM3

Additional MCM3 Products

Product Documents for MCM3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MCM3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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